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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated KAP1 Monoclonal antibody

KAP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B9G12

Cat no : CL488-66630

Synonyms

TRIM28, KRAB associated protein 1, KAP-1, KAP 1, EC:2.3.2.27

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated KAP1 Monoclonal anti (CL488-66630)

    IF Staining of HepG2 using CL488-66630

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated KAP1 antibody (CL488-66630, Clone: 1B9G12 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated KAP1 Monoclonal anti (CL488-66630)

    IF Staining of HepG2 using CL488-66630

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 KAP1 antibody (CL488-66630, Clone: 1B9G12 ) at dilution of 1:200, CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated KAP1 Monoclonal anti (CL488-66630)

    FC experiment of HeLa using CL488-66630

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human KAP1 (CL488-66630, Clone:1B9G12) (red), or 0.2 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66630 targets KAP1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen KAP1 fusion protein Ag7519 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 28
Calculated molecular weight 89 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC004978
Gene symbol KAP1
Gene ID (NCBI) 10155
RRIDAB_2883362
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 KAP1 antibody CL488-66630Download protocol
FC protocol for CL Plus 488 KAP1 antibody CL488-66630Download protocol
Standard Protocols
Click here to view our Standard Protocols