• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KAT2A/GCN5 Recombinant antibody, PBS Only (Capture)

KAT2A/GCN5 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242113C7

Cat no : 84669-2-PBS

Synonyms

General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48, EC:2.3.1.-

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using KAT2A/GCN5 Recombinant antibody (84669-2-RR)

    WB analysis using 84669-2-RR (same clone as 84669-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84669-2-RR (KAT2A/GCN5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84669-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KAT2A/GCN5 Recombinant antibody (84669-2-RR)

    WB analysis using 84669-2-RR (same clone as 84669-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84669-2-RR (KAT2A/GCN5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84669-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using KAT2A/GCN5 Recombinant antibody (84669-2-RR)

    IF Staining of MCF-7 using 84669-2-RR (same clone as 84669-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KAT2A/GCN5 antibody (84669-2-RR, Clone: 242113C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84669-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01463-1

    Cytometric bead array standard curve of MP01463-1

    Cytometric bead array standard curve of MP01463-1, KAT2A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84669-2-PBS. Detection antibody: 84669-3-PBS. Standard:Ag29005. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84669-2-RR

    Affinity and Kinetic Characterization of 84669-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84669-2-RR against Human KAT2A/GCN5 were performed. The affinity constant is 0.252 nM.

☆ 2/28（金）まで！最大30%オフ！ >> トライアルサイズもお得な一次抗体キャンペーン

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84669-2-PBS targets KAT2A/GCN5 as part of a matched antibody pair:

MP01463-1: 84669-2-PBS capture and 84669-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KAT2A/GCN5 fusion protein Ag29005 相同性解析による交差性が予測される生物種
Full Name K(lysine) acetyltransferase 2A
Calculated molecular weight 94 kDa
Observed molecular weight94 kDa
GenBank accession numberBC032743
Gene symbol KAT2A
Gene ID (NCBI) 2648
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.