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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KAT2A/GCN5 Recombinant antibody (84669-2-RR)

    WB analysis using 84669-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84669-2-RR (KAT2A/GCN5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KAT2A/GCN5 Recombinant antibody (84669-2-RR)

    WB analysis using 84669-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84669-2-RR (KAT2A/GCN5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using KAT2A/GCN5 Recombinant antibody (84669-2-RR)

    IF Staining of MCF-7 using 84669-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KAT2A/GCN5 antibody (84669-2-RR, Clone: 242113C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HEK-293 cells using KAT2A/GCN5 Recombinant antibody (84669-2-RR)

    FC experiment of HEK-293 using 84669-2-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug KAT2A/GCN5 Recombinant antibody (84669-2-RR, Clone:242113C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84669-2-RR

    Affinity and Kinetic Characterization of 84669-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84669-2-RR against Human KAT2A/GCN5 were performed. The affinity constant is 0.252 nM.

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KAT2A/GCN5 Recombinant antibody

KAT2A/GCN5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84669-2-RR
Clone No.242113C7

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

General control of amino acid synthesis protein 5-like 2, GCN5L2, GCN5, EC:2.3.1.48, EC:2.3.1.-

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, HeLa cells, MCF-7 cells, LNCaP cells, HSC-T6 cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84669-2-RR targets KAT2A/GCN5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KAT2A/GCN5 fusion protein Ag29005 相同性解析による交差性が予測される生物種
Full Name K(lysine) acetyltransferase 2A
Calculated molecular weight 94 kDa
Observed molecular weight94 kDa
GenBank accession numberBC032743
Gene Symbol KAT2A
Gene ID (NCBI) 2648
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92830
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GCN5, encoded by KAT2A, is a member of nuclear A-type histone acetyltransferase (HAT) which has direct impact on gene transcription. Additionally, GCN5 has roles in cell cycle regulation, DNA replication, and DNA repair, highlighting it as key player in the maintenance of genome stability. GCN5 is a coactivator of c-MYC oncogene protein and has oncogenic roles in various cancers.

Protocols

Product Specific Protocols
WB protocol for KAT2A/GCN5 antibody 84669-2-RRDownload protocol
IF protocol for KAT2A/GCN5 antibody 84669-2-RRDownload protocol
FC protocol for KAT2A/GCN5 antibody 84669-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols