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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KBTBD2 Monoclonal antibody (67895-1-Ig)

    WB analysis using 67895-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67895-1-Ig (KBTBD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of U2OS cells using KBTBD2 Monoclonal antibody (67895-1-Ig)

    WB analysis of U2OS using 67895-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67895-1-Ig (KBTBD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KBTBD2 Monoclonal antibody (67895-1-Ig)

    IF Staining of HeLa using 67895-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KBTBD2 antibody (67895-1-Ig, Clone: 2A9F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (blue).

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  • Featured Product
  • KD/KO Validated

KBTBD2 Monoclonal antibody

KBTBD2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67895-1-Ig
Clone No.2A9F3
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

BKLHD1, KBTBD2, KIAA1489

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, U2OS cells, HeLa cells, K-562 cells, Jurkat cells, HSC-T6 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67895-1-Ig targets KBTBD2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KBTBD2 fusion protein Ag4933 相同性解析による交差性が予測される生物種
Full Name kelch repeat and BTB (POZ) domain containing 2
Calculated molecular weight 622 aa, 71 kDa
Observed molecular weight60-70 kDa
GenBank accession numberBC047107
Gene Symbol KBTBD2
Gene ID (NCBI) 25948
RRIDAB_2918651
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8IY47
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kelch repeat and BTB domain containing 2(KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85α-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85α protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling (PMID:27708159). However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization (PMID:29331106). In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity(PMID:15623525, 29331106).

Protocols

Product Specific Protocols
WB protocol for KBTBD2 antibody 67895-1-IgDownload protocol
IF protocol for KBTBD2 antibody 67895-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Pathol Res Pract

KBTBD2 promotes proliferation and migration of gastric cancer via activating EGFR signaling pathway

Authors - Jishuang Ding
  • KD Validated
View Article