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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated KBTBD2 Monoclonal antibody

KBTBD2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A9F3

Cat no : CL594-67895

Synonyms

BKLHD1, KBTBD2, KIAA1489

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated KBTBD2 Monoclonal antibody (CL594-67895)

    IF Staining of HeLa using CL594-67895

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 KBTBD2 antibody (CL594-67895, Clone: 2A9F3 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67895 targets KBTBD2 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KBTBD2 fusion protein Ag4933 相同性解析による交差性が予測される生物種
Full Name kelch repeat and BTB (POZ) domain containing 2
Calculated molecular weight 622 aa, 71 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC047107
Gene symbol KBTBD2
Gene ID (NCBI) 25948
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Kelch repeat and BTB domain containing 2(KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85α-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85α protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling (PMID:27708159). However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization (PMID:29331106). In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity(PMID:15623525, 29331106).

Protocols

Product Specific Protocols
IF protocol for CL594 KBTBD2 antibody CL594-67895Download protocol
Standard Protocols
Click here to view our Standard Protocols