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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated KCC2/SLC12A5 Polyclonal antibody

KCC2/SLC12A5 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28724

Synonyms

hKCC2, K Cl cotransporter 2, KCC2, KIAA1176, Neuronal K Cl cotransporter, SLC12A5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using CoraLite® Plus 488-conjugated KCC2/SLC12A5 Polyclo (CL488-28724)

    IF Staining of mouse cerebellum using CL488-28724

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite® Plus 488 KCC2/SLC12A5 antibody (CL488-28724) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inmouse cerebellum tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28724 targets KCC2/SLC12A5 in IF-P applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCC2/SLC12A5 fusion protein Ag30203 相同性解析による交差性が予測される生物種
Full Name solute carrier family 12 (potassium-chloride transporter), member 5
Observed molecular weight130-150 kDa
GenBank accession numberBC132670
Gene symbol KCC2
Gene ID (NCBI) 57468
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KCC2, encoded by SLC12A5, is a neuron-specific K+-Cl- cotransporter that is essential for Cl- homeostasis and inhibitory synaptic transmission in brain. KCC2 is expressed at high levels in neurons throughout the nervous system. KCC2 deletion caused animal death. Alterations of KCC2 have been linked to neuropathic pain, temporal lobe epilepsy, and other diseases.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 KCC2/SLC12A5 antibody CL488-28724Download protocol
Standard Protocols
Click here to view our Standard Protocols