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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KCNH7 Recombinant antibody (83674-1-RR)

    WB analysis using 83674-1-RR (same clone as 83674-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83674-1-RR (KCNH7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83674-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using KCNH7 Recombinant antibody (83674-1-RR)

    WB analysis of pig brain using 83674-1-RR (same clone as 83674-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 83674-1-RR (KCNH7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83674-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KCNH7 Recombinant antibody (83674-1-RR)

    IHC staining of mouse brain using 83674-1-RR (same clone as 83674-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83674-1-RR (KCNH7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83674-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KCNH7 Recombinant antibody (83674-1-RR)

    IHC staining of mouse brain using 83674-1-RR (same clone as 83674-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83674-1-RR (KCNH7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83674-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00649-1

    Cytometric bead array standard curve of MP00649-1

    Cytometric bead array standard curve of MP00649-1, KCNH7 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83674-1-PBS. Detection antibody: 83674-2-PBS. Standard: Ag4532. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00649-2

    Cytometric bead array standard curve of MP00649-2

    Cytometric bead array standard curve of MP00649-2, KCNH7 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83674-4-PBS. Detection antibody: 83674-1-PBS. Standard: Ag4532. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00649-3

    Cytometric bead array standard curve of MP00649-3

    Cytometric bead array standard curve of MP00649-3, KCNH7 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83674-1-PBS. Detection antibody: 83674-3-PBS. Standard: Ag4532. Range: 1.25-80 ng/mL.

  • Affinity and Kinetic Characterization of 83674-1-RR

    Affinity and Kinetic Characterization of 83674-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83674-1-RR against Human KCNH7 were performed. The affinity constant is below 1 pM.

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KCNH7 Recombinant antibody, PBS Only (Capture/Detector)

KCNH7 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 83674-1-PBS
Clone No.240644B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

ERG-3, ERG3, ERG 3, Eag-related protein 3, Eag related protein 3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83674-1-PBS targets KCNH7 as part of a matched antibody pair:

MP00649-1: 83674-1-PBS capture and 83674-2-PBS detection (validated in Cytometric bead array)

MP00649-2: 83674-4-PBS capture and 83674-1-PBS detection (validated in Cytometric bead array)

MP00649-3: 83674-1-PBS capture and 83674-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KCNH7 fusion protein Ag4532 相同性解析による交差性が予測される生物種
Full Name potassium voltage-gated channel, subfamily H (eag-related), member 7
Calculated molecular weight 1196 aa, 135 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC035815
Gene Symbol KCNH7
Gene ID (NCBI) 90134
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NS40
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Potassium voltage-gated channel subfamily H member 7 is a protein that in humans is encoded by the KCNH7 gene. The protein encoded by this gene is a voltage-gated potassium channel subunit. KCNH7 is pore-forming (alpha) subunit of voltage-gated potassium channel. Channel properties may be modulated by cAMP and subunit assembly.