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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KCNH7 Recombinant antibody (83674-1-RR)

    WB analysis using 83674-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83674-1-RR (KCNH7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using KCNH7 Recombinant antibody (83674-1-RR)

    WB analysis of pig brain using 83674-1-RR

    pig brain tissue were subjected to SDS PAGE followed by western blot with 83674-1-RR (KCNH7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KCNH7 Recombinant antibody (83674-1-RR)

    IHC staining of mouse brain using 83674-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83674-1-RR (KCNH7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KCNH7 Recombinant antibody (83674-1-RR)

    IHC staining of mouse brain using 83674-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83674-1-RR (KCNH7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83674-1-RR

    Affinity and Kinetic Characterization of 83674-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83674-1-RR against Human KCNH7 were performed. The affinity constant is below 1 pM.

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KCNH7 Recombinant antibody

KCNH7 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83674-1-RR
Clone No.240644B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

ERG-3, ERG3, ERG 3, Eag-related protein 3, Eag related protein 3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, pig brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83674-1-RR targets KCNH7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KCNH7 fusion protein Ag4532 相同性解析による交差性が予測される生物種
Full Name potassium voltage-gated channel, subfamily H (eag-related), member 7
Calculated molecular weight 1196 aa, 135 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC035815
Gene Symbol KCNH7
Gene ID (NCBI) 90134
RRIDAB_3671279
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NS40
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Potassium voltage-gated channel subfamily H member 7 is a protein that in humans is encoded by the KCNH7 gene. The protein encoded by this gene is a voltage-gated potassium channel subunit. KCNH7 is pore-forming (alpha) subunit of voltage-gated potassium channel. Channel properties may be modulated by cAMP and subunit assembly.

Protocols

Product Specific Protocols
WB protocol for KCNH7 antibody 83674-1-RRDownload protocol
IHC protocol for KCNH7 antibody 83674-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols