KChIP1 Monoclonal antibody
KChIP1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
3D6C1
Cat no : 66439-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human cerebellum tissue, fetal human brain tissue, human spleen tissue |
| Positive IHC detected in | human brain tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66439-1-Ig targets KChIP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | KChIP1 fusion protein Ag5494 相同性解析による交差性が予測される生物種 |
| Full Name | Kv channel interacting protein 1 |
| Calculated molecular weight | 227 aa, 27 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC050375 |
| Gene symbol | KCNIP1 |
| Gene ID (NCBI) | 30820 |
| RRID | AB_2881809 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human K(v) channel interacting protein 1 (KCHIP1) is a new member of the neural calcium binding protein superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. KChIP1 is a neuronal calcium sensor protein that is predominantly expressed at GABAergic synapses and it has been related with modulation of K(+) channels, GABAergic transmission and cell death.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KChIP1 antibody 66439-1-Ig | Download protocol |
| IHC protocol for KChIP1 antibody 66439-1-Ig | Download protocol |
| IF protocol for KChIP1 antibody 66439-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
