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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KChIP1 Monoclonal antibody, PBS Only

KChIP1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D6C1

Cat no : 66439-1-PBS

Synonyms

KCHIP1, KCNIP1, VABP, Vesicle APC binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of human cerebellum tissue using KChIP1 Monoclonal antibody (66439-1-Ig)

    WB analysis of human cerebellum using 66439-1-Ig (same clone as 66439-1-PBS)

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using KChIP1 Monoclonal antibody (66439-1-Ig)

    WB analysis of fetal human brain using 66439-1-Ig (same clone as 66439-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using KChIP1 Monoclonal antibody (66439-1-Ig)

    WB analysis of human spleen using 66439-1-Ig (same clone as 66439-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using KChIP1 Monoclonal antibody (66439-1-Ig)

    IHC staining of human brain using 66439-1-Ig (same clone as 66439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using KChIP1 Monoclonal antibody (66439-1-Ig)

    IHC staining of human brain using 66439-1-Ig (same clone as 66439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using KChIP1 Monoclonal antibody (66439-1-Ig)

    IHC staining of human heart using 66439-1-Ig (same clone as 66439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using KChIP1 Monoclonal antibody (66439-1-Ig)

    IHC staining of human heart using 66439-1-Ig (same clone as 66439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KChIP1 Monoclonal antibody (66439-1-Ig)

    IF Staining of mouse brain using 66439-1-Ig (same clone as 66439-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KChIP1 antibody (66439-1-Ig, Clone: 3D6C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KChIP1 Monoclonal antibody (66439-1-Ig)

    IF Staining of mouse brain using 66439-1-Ig (same clone as 66439-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KChIP1 antibody (66439-1-Ig, Clone: 3D6C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66439-1-PBS targets KChIP1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KChIP1 fusion protein Ag5494 相同性解析による交差性が予測される生物種
Full Name Kv channel interacting protein 1
Calculated molecular weight 227 aa, 27 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC050375
Gene symbol KCNIP1
Gene ID (NCBI) 30820
RRIDAB_2881809
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Human K(v) channel interacting protein 1 (KCHIP1) is a new member of the neural calcium binding protein superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. KChIP1 is a neuronal calcium sensor protein that is predominantly expressed at GABAergic synapses and it has been related with modulation of K(+) channels, GABAergic transmission and cell death.