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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KCNIP4 Monoclonal antibody

KCNIP4 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

5B1D12

Cat no : 60133-1-Ig

Synonyms

CALP, Calsenilin like protein, KCHIP4, KCNIP4

Validation Data Gallery

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  • Western Blot (WB) analysis of pig brain tissue using KCNIP4 Monoclonal antibody (60133-1-Ig)

    WB analysis of pig brain using 60133-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60133-1-Ig (KCNIP4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using KCNIP4 Monoclonal antibody (60133-1-Ig)

    WB analysis of rat brain using 60133-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 60133-1-Ig (KCNIP4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using KCNIP4 Monoclonal antibody (60133-1-Ig)

    WB analysis of human brain using 60133-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 60133-1-Ig (KCNIP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using KCNIP4 Monoclonal antibody (60133-1-Ig)

    IHC staining of human brain using 60133-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60133-1-Ig (KCNIP4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using KCNIP4 Monoclonal antibody (60133-1-Ig)

    IHC staining of human brain using 60133-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60133-1-Ig (KCNIP4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KCNIP4 Monoclonal antibody (60133-1-Ig)

    IF Staining of mouse brain using 60133-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KCNIP4 antibody (60133-1-Ig, Clone: 5B1D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KCNIP4 Monoclonal antibody (60133-1-Ig)

    IF Staining of mouse brain using 60133-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KCNIP4 antibody (60133-1-Ig, Clone: 5B1D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inpig brain tissue, human brain tissue, rat brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60133-1-Ig targets KCNIP4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, pig, mouse samples.

Tested Reactivity human, rat, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KCNIP4 fusion protein Ag4863 相同性解析による交差性が予測される生物種
Full Name Kv channel interacting protein 4
Calculated molecular weight 250 aa, 28 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC032520
Gene symbol KCNIP4
Gene ID (NCBI) 80333
RRIDAB_2130271
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KCNIP4 is a member of the family of voltage-gated potassium (Kv) channel-interacting proteins (KCNIPs), which belong to the recoverin branch of the EF-hand superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium.

Protocols

Product Specific Protocols
WB protocol for KCNIP4 antibody 60133-1-IgDownload protocol
IHC protocol for KCNIP4 antibody 60133-1-IgDownload protocol
IF protocol for KCNIP4 antibody 60133-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols