Validation Data Gallery
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Tested Applications
| Positive WB detected in | pig brain tissue, human brain tissue, rat brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 2 publications below |
Product Information
60133-1-Ig targets KCNIP4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, pig, mouse samples.
| Tested Reactivity | human, rat, pig, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | KCNIP4 fusion protein Ag4863 相同性解析による交差性が予測される生物種 |
| Full Name | Kv channel interacting protein 4 |
| Calculated molecular weight | 250 aa, 28 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC032520 |
| Gene Symbol | KCNIP4 |
| Gene ID (NCBI) | 80333 |
| RRID | AB_2130271 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q6PIL6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KCNIP4 is a member of the family of voltage-gated potassium (Kv) channel-interacting proteins (KCNIPs), which belong to the recoverin branch of the EF-hand superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KCNIP4 antibody 60133-1-Ig | Download protocol |
| IHC protocol for KCNIP4 antibody 60133-1-Ig | Download protocol |
| IF protocol for KCNIP4 antibody 60133-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
iScience Single-nucleus RNA sequencing-based construction of a hippocampal neuron atlas in mice with epileptic cognitive impairment | ||
bioRxiv Molecular Signatures of Resilience to Alzheimer's Disease in Neocortical Layer 4 Neurons |