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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KCNIP4 Monoclonal antibody, PBS Only

KCNIP4 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5B1D12

Cat no : 60133-1-PBS

Synonyms

CALP, Calsenilin like protein, KCHIP4, KCNIP4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of pig brain tissue using KCNIP4 Monoclonal antibody (60133-1-Ig)

    WB analysis of pig brain using 60133-1-Ig (same clone as 60133-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60133-1-Ig (KCNIP4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using KCNIP4 Monoclonal antibody (60133-1-Ig)

    WB analysis of rat brain using 60133-1-Ig (same clone as 60133-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 60133-1-Ig (KCNIP4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60133-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using KCNIP4 Monoclonal antibody (60133-1-Ig)

    WB analysis of human brain using 60133-1-Ig (same clone as 60133-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 60133-1-Ig (KCNIP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using KCNIP4 Monoclonal antibody (60133-1-Ig)

    IHC staining of human brain using 60133-1-Ig (same clone as 60133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60133-1-Ig (KCNIP4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60133-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using KCNIP4 Monoclonal antibody (60133-1-Ig)

    IHC staining of human brain using 60133-1-Ig (same clone as 60133-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60133-1-Ig (KCNIP4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60133-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KCNIP4 Monoclonal antibody (60133-1-Ig)

    IF Staining of mouse brain using 60133-1-Ig (same clone as 60133-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KCNIP4 antibody (60133-1-Ig, Clone: 5B1D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60133-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using KCNIP4 Monoclonal antibody (60133-1-Ig)

    IF Staining of mouse brain using 60133-1-Ig (same clone as 60133-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KCNIP4 antibody (60133-1-Ig, Clone: 5B1D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60133-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60133-1-PBS targets KCNIP4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, rat, pig, mouse samples.

Tested Reactivity human, rat, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KCNIP4 fusion protein Ag4863 相同性解析による交差性が予測される生物種
Full Name Kv channel interacting protein 4
Calculated molecular weight 250 aa, 28 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC032520
Gene symbol KCNIP4
Gene ID (NCBI) 80333
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

KCNIP4 is a member of the family of voltage-gated potassium (Kv) channel-interacting proteins (KCNIPs), which belong to the recoverin branch of the EF-hand superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium.