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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using KCNJ15 Recombinant antibody (83257-5-RR)

    WB analysis of A549 using 83257-5-RR (same clone as 83257-5-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 83257-5-RR (KCNJ15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83257-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KCNJ15 Recombinant antibody (83257-5-RR)

    IHC staining of mouse brain using 83257-5-RR (same clone as 83257-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83257-5-RR (KCNJ15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83257-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using KCNJ15 Recombinant antibody (83257-5-RR)

    FC experiment of HepG2 using 83257-5-RR (same clone as 83257-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug KCNJ15 Recombinant antibody (83257-5-RR, Clone:240141C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83257-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83257-5-RR

    Affinity and Kinetic Characterization of 83257-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83257-5-RR against Human KCNJ15 were performed. The affinity constant is 0.221 nM.

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KCNJ15 Recombinant antibody, PBS Only

KCNJ15 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83257-5-PBS
Clone No.240141C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

KIR4.2, KIR1.3, KCNJ14, IRKK, ATP-sensitive inward rectifier potassium channel 15

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83257-5-PBS targets KCNJ15 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KCNJ15 fusion protein Ag34655 相同性解析による交差性が予測される生物種
Full Name potassium inwardly-rectifying channel, subfamily J, member 15
Calculated molecular weight 375 aa, 43 kDa
Observed molecular weight43 kDa
GenBank accession numberBC013327
Gene Symbol KCNJ15
Gene ID (NCBI) 3772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ99712
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

KCNJ15, also named as KIR1.3, KIR4.2, and IRKK, is a multi-pass membrane protein belonging to the inwardly-rectifying potassium channel (KIR) family. KIR channels perform functions as diverse as the regulation of resting membrane potential, maintenance of potassium ions homeostasis, control of heart rate and hormone secretion. Cloned from human kidney, the KCNJ15 gene is localized on chromosome 21 in the Down syndrome chromosome region 1 (DCR1) and has been identified as a type 2 diabetes-associated risk gene. The gene is most readily expressed in the pancreas and kidney and less in the lung. (PMID: 8995301; 9299242; 20085713)