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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KCNJ15 Recombinant antibody

KCNJ15 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240141C3

Cat no : 83257-5-RR

Synonyms

IRKK, KCNJ14, KCNJ15, KIR1.3, KIR4.2

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using KCNJ15 Recombinant antibody (83257-5-RR)

    WB analysis of A549 using 83257-5-RR

    A549 cells were subjected to SDS PAGE followed by western blot with 83257-5-RR (KCNJ15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83257-5-RR is KCNJ15 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KCNJ15 fusion protein Ag34655 相同性解析による交差性が予測される生物種
Full Name potassium inwardly-rectifying channel, subfamily J, member 15
Calculated molecular weight 375 aa, 43 kDa
Observed molecular weight43 kDa
GenBank accession numberBC013327
Gene symbol KCNJ15
Gene ID (NCBI) 3772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KCNJ15, also named as KIR1.3, KIR4.2, and IRKK, is a multi-pass membrane protein belonging to the inwardly-rectifying potassium channel (KIR) family. KIR channels perform functions as diverse as the regulation of resting membrane potential, maintenance of potassium ions homeostasis, control of heart rate and hormone secretion. Cloned from human kidney, the KCNJ15 gene is localized on chromosome 21 in the Down syndrome chromosome region 1 (DCR1) and has been identified as a type 2 diabetes-associated risk gene. The gene is most readily expressed in the pancreas and kidney and less in the lung. (PMID: 8995301; 9299242; 20085713)

Protocols

Product Specific Protocols
WB protocol for KCNJ15 antibody 83257-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols