Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HepG2 cells, HEK-293 cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
| FC | See 1 publications below |
Product Information
23271-1-AP targets KCNN4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KCNN4 fusion protein Ag19774 相同性解析による交差性が予測される生物種 |
| Full Name | potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4 |
| Calculated molecular weight | 427 aa, 48 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC015337 |
| Gene Symbol | KCNN4 |
| Gene ID (NCBI) | 3783 |
| RRID | AB_2879243 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | O15554 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The intermediate conductance calcium-activated potassium channel KCNN4, also known as KCa3.1, IK1, or SK4, is a member of the potassium channel KCNN family. KCNN4 is expressed in T cells, mast cells, macrophages, erythrocytes, vascular smooth muscle cells, airway smooth muscle cells, intestinal and airway epithelial cells (PMID: 19681865; 21493782). Functionally, KCNN4 plays a key role in calcium-dependent cell functions, such as proliferation, activation, and migration, in a broad range of cell types (PMID: 21493782).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KCNN4 antibody 23271-1-AP | Download protocol |
| IF protocol for KCNN4 antibody 23271-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Int KCNN4 may weaken anti-tumor immune response via raising Tregs and diminishing resting mast cells in clear cell renal cell carcinoma. | ||
Cancer Biomark KCNN4 promotes the progression of lung adenocarcinoma by activating the AKT and ERK signaling pathways
| ||
Front Physiol New KCNN4 Variants Associated With Anemia: Stomatocytosis Without Erythrocyte Dehydration | ||
Cardiovasc Ther KCa3.1 Promotes Proinflammatory Exosome Secretion by Activating AKT/Rab27a in Atrial Myocytes during Rapid Pacing | ||
Biochem Biophys Res Commun Integrated expression profiling of potassium channels identifys KCNN4 as a prognostic biomarker of pancreatic cancer. | ||
Nat Commun Phospholipid scrambling induced by an ion channel/metabolite transporter complex |