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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite® Plus 488-conjugated KCNN4 Polyclonal ant (CL488-23271)

    IF Staining of HEK-293 using CL488-23271

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 KCNN4 antibody (CL488-23271) at dilution of 1:200.

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated KCNN4 Polyclonal antibody

KCNN4 Polyclonal Antibody for IF/ICC
Cat No. CL488-23271

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

hIKCa1, hKCa4, hSK4, IK1, IKCA1, KCa3.1, KCA4, KCNN4, Putative Gardos channel, SK4, SKCa 4, SKCa4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-23271 targets KCNN4 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNN4 fusion protein Ag19774 相同性解析による交差性が予測される生物種
Full Name potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4
Calculated molecular weight 427 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC015337
Gene Symbol KCNN4
Gene ID (NCBI) 3783
RRIDAB_3672753
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15554
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The intermediate conductance calcium-activated potassium channel KCNN4, also known as KCa3.1, IK1, or SK4, is a member of the potassium channel KCNN family. KCNN4 is expressed in T cells, mast cells, macrophages, erythrocytes, vascular smooth muscle cells, airway smooth muscle cells, intestinal and airway epithelial cells (PMID: 19681865; 21493782). Functionally, KCNN4 plays a key role in calcium-dependent cell functions, such as proliferation, activation, and migration, in a broad range of cell types (PMID: 21493782).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 KCNN4 antibody CL488-23271Download protocol
Standard Protocols
Click here to view our Standard Protocols