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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using KCNN4 Polyclonal antibody (23271-1-AP)

    WB analysis of HEK-293 using 23271-1-AP (same clone as 23271-1-PBS)

    WB result of KCNN4 antibody (23271-1-AP, 1:4000) with si-control and si-KCNN4 transfected HEK293 cells. This data was developed using the same antibody clone with 23271-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KCNN4 Polyclonal antibody (23271-1-AP)

    WB analysis using 23271-1-AP (same clone as 23271-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23271-1-AP (KCNN4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23271-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using KCNN4 Polyclonal antibody (23271-1-AP)

    WB analysis of HepG2 using 23271-1-AP (same clone as 23271-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23271-1-AP (KCNN4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using KCNN4 Polyclonal antibody (23271-1-AP)

    IF Staining of HEK-293 using 23271-1-AP (same clone as 23271-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using KCNN4 antibody (23271-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23271-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

KCNN4 Polyclonal antibody, PBS Only

KCNN4 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 23271-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23271-1-PBS targets KCNN4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNN4 fusion protein Ag19774 相同性解析による交差性が予測される生物種
Full Name potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4
Calculated molecular weight 427 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC015337
Gene Symbol KCNN4
Gene ID (NCBI) 3783
RRIDAB_2879243
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDO15554
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The intermediate conductance calcium-activated potassium channel KCNN4, also known as KCa3.1, IK1, or SK4, is a member of the potassium channel KCNN family. KCNN4 is expressed in T cells, mast cells, macrophages, erythrocytes, vascular smooth muscle cells, airway smooth muscle cells, intestinal and airway epithelial cells (PMID: 19681865; 21493782). Functionally, KCNN4 plays a key role in calcium-dependent cell functions, such as proliferation, activation, and migration, in a broad range of cell types (PMID: 21493782).