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  • Featured Product
  • KD/KO Validated

KCNN4 Polyclonal antibody, PBS Only

KCNN4 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23271-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using KCNN4 Polyclonal antibody (23271-1-AP)

    WB analysis of HEK-293 using 23271-1-AP (same clone as 23271-1-PBS)

    WB result of KCNN4 antibody (23271-1-AP, 1:4000) with si-control and si-KCNN4 transfected HEK293 cells. This data was developed using the same antibody clone with 23271-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KCNN4 Polyclonal antibody (23271-1-AP)

    WB analysis using 23271-1-AP (same clone as 23271-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23271-1-AP (KCNN4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23271-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using KCNN4 Polyclonal antibody (23271-1-AP)

    WB analysis of HepG2 using 23271-1-AP (same clone as 23271-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23271-1-AP (KCNN4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23271-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using KCNN4 Polyclonal antibody (23271-1-AP)

    IF Staining of HEK-293 using 23271-1-AP (same clone as 23271-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using KCNN4 antibody (23271-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23271-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23271-1-PBS targets KCNN4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KCNN4 fusion protein Ag19774 相同性解析による交差性が予測される生物種
Full Name potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4
Calculated molecular weight 427 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC015337
Gene symbol KCNN4
Gene ID (NCBI) 3783
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The intermediate conductance calcium-activated potassium channel KCNN4, also known as KCa3.1, IK1, or SK4, is a member of the potassium channel KCNN family. KCNN4 is expressed in T cells, mast cells, macrophages, erythrocytes, vascular smooth muscle cells, airway smooth muscle cells, intestinal and airway epithelial cells (PMID: 19681865; 21493782). Functionally, KCNN4 plays a key role in calcium-dependent cell functions, such as proliferation, activation, and migration, in a broad range of cell types (PMID: 21493782).