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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KDM3A,JMJD1A Recombinant antibody, PBS Only (Capture)

KDM3A,JMJD1A Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240532G11

Cat no : 83571-2-PBS

Synonyms

KDM3A, JmjC domain-containing histone demethylation protein 2A, JHMD2A, JHDM2A, EC:1.14.11.65

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KDM3A,JMJD1A Recombinant antibody (83571-2-RR)

    WB analysis using 83571-2-RR (same clone as 83571-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83571-2-RR (KDM3A,JMJD1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83571-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KDM3A,JMJD1A Recombinant antibody (83571-2-RR)

    IF Staining of HepG2 using 83571-2-RR (same clone as 83571-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KDM3A,JMJD1A antibody (83571-2-RR, Clone: 240532G11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83571-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using KDM3A,JMJD1A Recombinant antibody (83571-2-RR)

    FC experiment of HEK-293 using 83571-2-RR (same clone as 83571-2-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug KDM3A,JMJD1A Recombinant antibody (83571-2-RR, Clone:240532G11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83571-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00565-1

    Cytometric bead array standard curve of MP00565-1

    Cytometric bead array standard curve of MP00565-1, KDM3A,JMJD1A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83571-2-PBS. Detection antibody: 83571-4-PBS. Standard: Ag28687. Range: 1.56-100 ng/mL.

  • Cytometric bead array standard curve of MP00565-2

    Cytometric bead array standard curve of MP00565-2

    Cytometric bead array standard curve of MP00565-2, KDM3A,JMJD1A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83571-2-PBS. Detection antibody: 83571-5-PBS. Standard: Ag28687. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83571-2-RR

    Affinity and Kinetic Characterization of 83571-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83571-2-RR against Human KDM3A,JMJD1A were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83571-2-PBS targets KDM3A,JMJD1A as part of a matched antibody pair:

MP00565-1: 83571-2-PBS capture and 83571-4-PBS detection (validated in Cytometric bead array)

MP00565-2: 83571-2-PBS capture and 83571-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KDM3A,JMJD1A fusion protein Ag28687 相同性解析による交差性が予測される生物種
Full Name lysine (K)-specific demethylase 3A
Calculated molecular weight 150 kDa
GenBank accession numberBC038297
Gene symbol KDM3A/JMJD1A
Gene ID (NCBI) 55818
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.