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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KDM3A,JMJD1A Recombinant antibody (83571-2-RR)

    WB analysis using 83571-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83571-2-RR (KDM3A,JMJD1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using KDM3A/JMJD1A Recombinant antibody (83571-2-RR)

    IHC staining of human ovarian cancer using 83571-2-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83571-2-RR (KDM3A,JMJD1A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KDM3A/JMJD1A Recombinant antibody (83571-2-RR)

    IF Staining of HepG2 using 83571-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KDM3A,JMJD1A antibody (83571-2-RR, Clone: 240532G11 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HEK-293 cells using KDM3A/JMJD1A Recombinant antibody (83571-2-RR)

    FC experiment of HEK-293 using 83571-2-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug KDM3A,JMJD1A Recombinant antibody (83571-2-RR, Clone:240532G11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83571-2-RR

    Affinity and Kinetic Characterization of 83571-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83571-2-RR against Human KDM3A,JMJD1A were performed. The affinity constant is below 1 pM.

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KDM3A/JMJD1A Recombinant antibody

KDM3A/JMJD1A Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83571-2-RR
Clone No.240532G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

KDM3A, KDM3A,JMJD1A, JmjC domain-containing histone demethylation protein 2A, JHMD2A, JHDM2A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells, SKOV-3 cells, HCT 116 cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83571-2-RR targets KDM3A/JMJD1A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KDM3A/JMJD1A fusion protein Ag28687 相同性解析による交差性が予測される生物種
Full Name lysine (K)-specific demethylase 3A
Calculated molecular weight 150 kDa
Observed molecular weight140-150 kDa
GenBank accession numberBC038297
Gene Symbol KDM3A/JMJD1A
Gene ID (NCBI) 55818
RRIDAB_3671184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4C1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KDM3A, also name as JMJD1A, JHDM2A, JMJD1, JMJD1A, KIAA0742 and TSGA, belongs to the JHDM2 histone demethylase family. It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. It is involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. It is involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. (PubMed:16603237), JMJD1A is a useful biomarker for hypoxic tumor cells and a prognostic marker that could be a promising therapeutic target against CRC.

Protocols

Product Specific Protocols
WB protocol for KDM3A/JMJD1A antibody 83571-2-RRDownload protocol
IHC protocol for KDM3A/JMJD1A antibody 83571-2-RRDownload protocol
IF protocol for KDM3A/JMJD1A antibody 83571-2-RRDownload protocol
FC protocol for KDM3A/JMJD1A antibody 83571-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols