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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KDM4A Polyclonal antibody (29977-1-AP)

    WB analysis using 29977-1-AP (same clone as 29977-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29977-1-AP (KDM4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29977-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using KDM4A Polyclonal antibody (29977-1-AP)

    WB analysis of mouse lung using 29977-1-AP (same clone as 29977-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 29977-1-AP (KDM4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29977-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using KDM4A Polyclonal antibody (29977-1-AP)

    IP experiment of MCF-7 using 29977-1-AP (same clone as 29977-1-PBS)

    IP result of anti-KDM4A (IP:29977-1-AP, 4ug; Detection:29977-1-AP 1:1500) with MCF-7 cells lysate 2120 ug. This data was developed using the same antibody clone with 29977-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A549 cells using KDM4A Polyclonal antibody (29977-1-AP)

    IP experiment of A549 using 29977-1-AP (same clone as 29977-1-PBS)

    IP result of anti-KDM4A (IP:29977-1-AP, 4ug; Detection:29977-1-AP 1:1500) with A549 cells lysate 800 ug. This data was developed using the same antibody clone with 29977-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using KDM4A Polyclonal antibody (29977-1-AP)

    FC experiment of A549 using 29977-1-AP (same clone as 29977-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human KDM4A (29977-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 29977-1-PBS in a different storage buffer formulation.

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KDM4A Polyclonal antibody, PBS Only

KDM4A Polyclonal Antibody for WB, FC (Intra), IP, Indirect ELISA
Cat No. 29977-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29977-1-PBS targets KDM4A in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KDM4A fusion protein Ag31992 相同性解析による交差性が予測される生物種
Full Name lysine (K)-specific demethylase 4A
Calculated molecular weight 1064 aa, 121 kDa
Observed molecular weight130-150 kDa
GenBank accession numberBC002558
Gene Symbol KDM4A
Gene ID (NCBI) 9682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75164
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

KDM4A also named as JHDM3A, JMJD2, or KIAA0677 is a 1066 amino acid protein, which contains one JmjC domain, two Tudor domains and two PHD-type zinc fingers. KDM4A localizes in nucleus and belongs to the JHDM3 histone demethylase family. KDM4A is histone de-methylase that specifically demethylates Lys 9 and Lys 36 residues of Histone H3, thereby playing a central role in histone code. JMJD2A demethylation of lysine residues will generate formaldehyde and succinate. KDM4A also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. JMJD2A is a ubiquitously expressed nuclear protein. KDM4A exists as two isoforms. The short isoform of KDM4A in which the N-terminal demethylase domains has been deleted. The molecular weight of short isoform is 55Da.