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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VEGFR2/KDR Recombinant antibody, PBS Only

VEGFR2/KDR Recombinant Antibody for WB, IHC, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230238D7

Cat no : 83049-4-PBS

Synonyms

KDR, VEGFR2, Protein-tyrosine kinase receptor flk-1, FLK-1, EC:2.7.10.1

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using VEGFR2/KDR Recombinant antibody (83049-4-RR)

    WB analysis of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

    human placenta tissue was subjected to SDS PAGE followed by western blot with 83049-4-RR (KDR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using VEGFR2/KDR Recombinant antibody (83049-4-RR)

    IHC staining of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (KDR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using VEGFR2/KDR Recombinant antibody (83049-4-RR)

    IHC staining of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (KDR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HUVEC cells using VEGFR2/KDR Recombinant antibody (83049-4-RR)

    FC experiment of HUVEC using 83049-4-RR (same clone as 83049-4-PBS)

    1x10^6 HUVEC cells were surface stained with 0.25 ug VEGF Receptor 2 Recombinant antibody (83049-4-RR, Clone:230238D7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83049-4-RR

    Affinity and Kinetic Characterization of 83049-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83049-4-RR against Human KDR were performed. The affinity constant is 4.71 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83049-4-PBS targets VEGFR2/KDR in WB, IHC, FC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name kinase insert domain receptor (a type III receptor tyrosine kinase)
Calculated molecular weight152kd
Observed molecular weight150 kDa, 230 kDa
GenBank accession numberNM_002253
Gene symbol KDR
Gene ID (NCBI) 3791
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.