Validation Data Gallery

Filter: AllWBIHCFCAffinity

WB analysis of human placenta using 83049-4-RR
human placenta tissue was subjected to SDS PAGE followed by western blot with 83049-4-RR (KDR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human placenta using 83049-4-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (KDR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 83049-4-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (KDR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human rectal cancer using 83049-4-RR
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 83049-4-RR (VEGFR2/KDR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 83049-4-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83049-4-RR (VEGFR2/KDR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 83049-4-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83049-4-RR (VEGFR2/KDR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human rectal cancer using 83049-4-RR
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 83049-4-RR (VEGFR2/KDR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HUVEC using 83049-4-RR
1x10^6 HUVEC cells were surface stained with 0.25 ug VEGF Receptor 2 Recombinant antibody (83049-4-RR, Clone:230238D7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.
Affinity and Kinetic Characterization of 83049-4-RR
Biolayer interferometry (BLl) kinetic assays of 83049-4-RR against Human KDR were performed. The affinity constant is 4.71 nM.

VEGFR2/KDR Recombinant antibody

VEGFR2/KDR Recombinant Antibody for WB, IHC, FC, ELISA

Cat No. 83049-4-RR

Clone No.230238D7

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC, ELISA

Synonyms

KDR, VEGFR2, Protein-tyrosine kinase receptor flk-1, FLK-1, EC:2.7.10.1

Formulation: PBS and Azide

PBS and Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human placenta tissue
Positive IHC detected in	human placenta tissue, human rectal cancer tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC detected in	HUVEC cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Flow Cytometry (FC)	FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

83049-4-RR targets VEGFR2/KDR in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Recombinant protein 相同性解析による交差性が予測される生物種
Full Name	kinase insert domain receptor (a type III receptor tyrosine kinase)
Calculated molecular weight	152kd
Observed molecular weight	150 kDa, 230 kDa
GenBank accession number	NM_002253
Gene Symbol	KDR
Gene ID (NCBI)	3791
RRID	AB_3670774
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P35968-1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.

Protocols

Product Specific Protocols
WB protocol for VEGFR2/KDR antibody 83049-4-RR	Download protocol
IHC protocol for VEGFR2/KDR antibody 83049-4-RR	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Biol Toxicol HNRNPA2B1 stabilizes NFATC3 levels to potentiate its combined actions with FOSL1 to mediate vasculogenic mimicry in GBM cells Authors - Hanting Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human placenta using 83049-4-RR

IHC staining of human placenta using 83049-4-RR