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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KEPI Polyclonal antibody (24933-1-AP)

    WB analysis using 24933-1-AP (same clone as 24933-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24933-1-AP (KEPI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24933-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KEPI Polyclonal antibody (24933-1-AP)

    WB analysis using 24933-1-AP (same clone as 24933-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24933-1-AP (KEPI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24933-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using KEPI Polyclonal antibody (24933-1-AP)

    WB analysis of HeLa using 24933-1-AP (same clone as 24933-1-PBS)

    WB result of KEPI antibody (24933-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KEPI transfected HeLa cells. This data was developed using the same antibody clone with 24933-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

KEPI Polyclonal antibody, PBS Only

KEPI Polyclonal Antibody for WB, Indirect ELISA
Cat No. 24933-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24933-1-PBS targets KEPI in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KEPI fusion protein Ag20645 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 14C
Calculated molecular weight 18 kDa
Observed molecular weight20-22 kDa
GenBank accession numberBC015773
Gene Symbol PPP1R14C
Gene ID (NCBI) 81706
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TAE6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PPP1R14C, found as a morphine‐regulated brain gene, encodes protein phosphatase 1 regulatory inhibitor subunit 14C, and is identified as a potent inhibitor of PP1. Horvath and colleagues reported that PPP1R14C inhibited the release of neurotransmitters and promoted neuronal exocytosis by retaining synaptosome associated protein 25 (SNAP25) at a phosphorylated state. Moreover, overexpression of PPP1R14C increased the phosphorylation of RB transcriptional co‐repressor 1 (RB1) to protect leukemic cells from chemotherapy.