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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated KGA-Specific Monoclo (CL488-66265)

    WB analysis using CL488-66265

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66265 (KGA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated KGA-Specific Monoclo (CL488-66265)

    IF Staining of mouse brain using CL488-66265

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 KGA-Specific antibody (CL488-66265, Clone: 1B11A11 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated KGA-Specific Monoclo (CL488-66265)

    IF Staining of mouse brain using CL488-66265

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 KGA-Specific antibody (CL488-66265, Clone: 1B11A11 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated KGA-Specific Monoclo (CL488-66265)

    IF Staining of mouse brain using CL488-66265

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 KGA-Specific antibody (CL488-66265, Clone: 1B11A11 ) at dilution of 1:200.

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CoraLite® Plus 488-conjugated KGA-Specific Monoclonal antibody

KGA-Specific Monoclonal Antibody for WB, IF-P
Cat No. CL488-66265
Clone No.1B11A11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF-P

Synonyms

AAD20, DKFZp686O15119, FLJ10358, GAC, GLS, GLS1, glutaminase, K glutaminase, KGA-Specific, KIAA0838, L glutamine amidohydrolase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, mouse brain tissue
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66265 targets KGA-Specific in WB, IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KGA-Specific fusion protein Ag24227 相同性解析による交差性が予測される生物種
Full Name glutaminase
Calculated molecular weight 669 aa, 73 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC038507
Gene Symbol GLS
Gene ID (NCBI) 2744
RRIDAB_3084228
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO94925
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID: 12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID: 447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 KGA-Specific antibody CL488-66265Download protocol
IF protocol for CL Plus 488 KGA-Specific antibody CL488-66265Download protocol
Standard Protocols
Click here to view our Standard Protocols