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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KHSRP Polyclonal antibody (55409-1-AP)

    WB analysis using 55409-1-AP (same clone as 55409-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55409-1-AP (KHSRP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55409-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using KHSRP Polyclonal antibody (55409-1-AP)

    WB analysis of HeLa using 55409-1-AP (same clone as 55409-1-PBS)

    WB result of KHSRP antibody (55409-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-KHSRP transfected HeLa cells. This data was developed using the same antibody clone with 55409-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KHSRP Polyclonal antibody (55409-1-AP)

    WB analysis using 55409-1-AP (same clone as 55409-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55409-1-AP (KHSRP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55409-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

KHSRP Polyclonal antibody, PBS Only

KHSRP Polyclonal Antibody for WB, Indirect ELISA
Cat No. 55409-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55409-1-PBS targets KHSRP in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name KH-type splicing regulatory protein
Calculated molecular weight 73 kDa
Observed molecular weight 75 kDa
GenBank accession numberNM_003685
Gene Symbol KHSRP
Gene ID (NCBI) 8570
RRIDAB_11182170
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92945
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.