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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Ki-67 Polyclonal antibody

Ki-67 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-27309

Synonyms

MKI67, KI67, Proliferation marker protein Ki-67, Ki 67, Antigen KI-67

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated KI67 Polyclonal antibody (CL594-27309)

    IF Staining of HeLa using CL594-27309

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 KI67 antibody (CL594-27309) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated KI67 Polyclonal antibody (CL594-27309)

    IF Staining of HeLa using CL594-27309

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 KI67 antibody (CL594-27309) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated KI67 Polyclonal antibody (CL594-27309)

    FC experiment of HeLa using CL594-27309

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human KI67 (CL594-27309) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of Ramos cells using CoraLite®594-conjugated KI67 Polyclonal antibody (CL594-27309)

    FC experiment of Ramos using CL594-27309

    1x10^6 Ramos cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human KI67 (CL594-27309)(red), or 0.8 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inRamos cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

CL594-27309 targets Ki-67 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ki-67 fusion protein Ag26266 相同性解析による交差性が予測される生物種
Full Name antigen identified by monoclonal antibody Ki-67
Calculated molecular weight 359 kDa
GenBank accession numberNM_002417
Gene symbol KI67
Gene ID (NCBI) 4288
RRIDAB_2919880
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

Protocols

Product Specific Protocols
IF protocol for CL594 Ki-67 antibody CL594-27309Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Oncol Rep

Zinc finger protein 180 induces an apoptotic phenotype by activating METTL14 transcriptional activity in colorectal cancer

Authors - Liang Xu
View Article