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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATG13 Monoclonal antibody

ATG13 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1C3A7

Cat no : 66708-1-Ig

Synonyms

KIAA0652, Autophagy-related protein 13, Autophagy related protein 13, 1C3A7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATG13 Monoclonal antibody (66708-1-Ig)

    WB analysis using 66708-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66708-1-Ig (ATG13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ATG13 Monoclonal antibody (66708-1-Ig)

    WB analysis of mouse brain using 66708-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BGC-823 cells using ATG13 Monoclonal antibody (66708-1-Ig)

    WB analysis of BGC-823 using 66708-1-Ig

    BGC-823 cells were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using ATG13 Monoclonal antibody (66708-1-Ig)

    WB analysis of NIH/3T3 using 66708-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using ATG13 Monoclonal antibody (66708-1-Ig)

    WB analysis of pig brain using 66708-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using ATG13 Monoclonal antibody (66708-1-Ig)

    WB analysis of rat brain using 66708-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66708-1-Ig (KIAA0652 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ATG13 Monoclonal antibody (66708-1-Ig)

    IHC staining of mouse testis using 66708-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66708-1-Ig (KIAA0652 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ATG13 Monoclonal antibody (66708-1-Ig)

    IHC staining of mouse testis using 66708-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66708-1-Ig (KIAA0652 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ATG13 Monoclonal antibody (66708-1-Ig)

    IHC staining of mouse brain using 66708-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66708-1-Ig (KIAA0652 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ATG13 Monoclonal antibody (66708-1-Ig)

    IHC staining of mouse brain using 66708-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66708-1-Ig (KIAA0652 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ATG13 Monoclonal antibody (66708-1-Ig)

    IF Staining of HeLa using 66708-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ATG13 antibody (66708-1-Ig, Clone: 1C3A7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using ATG13 Monoclonal antibody (66708-1-Ig)

    FC experiment of HeLa using 66708-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATG13 (66708-1-Ig, Clone:1C3A7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHEK-293 cells, BGC-823 cells, NIH/3T3 cells, pig brain tissue, rat brain tissue, mouse brain tissue, HeLa cells, Jurkat cells
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66708-1-Ig targets ATG13 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATG13 fusion protein Ag12968 相同性解析による交差性が予測される生物種
Full Name KIAA0652
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC001331
Gene symbol ATG13
Gene ID (NCBI) 9776
RRIDAB_2882060
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)

Protocols

Product Specific Protocols
WB protocol for ATG13 antibody 66708-1-IgDownload protocol
IHC protocol for ATG13 antibody 66708-1-IgDownload protocol
IF protocol for ATG13 antibody 66708-1-IgDownload protocol
FC protocol for ATG13 antibody 66708-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

Destabilization of TP53 by USP10 is essential for neonatal autophagy and survival

Authors - Hongchang Li
View Article
humanWB

Cancer Chemother Pharmacol

Avermectin B1 mediates antitumor activity and induces autophagy in osteosarcoma through the AMPK/ULK1 signaling pathway

Authors - Xiang Fei
View Article