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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated ATG13 Monoclonal antibody (CL594-66708)

    IF Staining of HeLa using CL594-66708

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 ATG13 antibody (CL594-66708, Clone: 1C3A7 ) at dilution of 1:200.

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CoraLite®594-conjugated ATG13 Monoclonal antibody

ATG13 Monoclonal Antibody for IF/ICC
Cat No. CL594-66708
Clone No.1C3A7

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

IF/ICC

Synonyms

ATG13, Autophagy related protein 13, FLJ20698, KIAA0652

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66708 targets ATG13 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ATG13 fusion protein Ag12968 相同性解析による交差性が予測される生物種
Full Name KIAA0652
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC001331
Gene Symbol ATG13
Gene ID (NCBI) 9776
RRIDAB_2923924
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75143
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)

Protocols

Product Specific Protocols
IF protocol for CL594 ATG13 antibody CL594-66708Download protocol
Standard Protocols
Click here to view our Standard Protocols