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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIAA1429 Monoclonal antibody

KIAA1429 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3F1A4

Cat no : 68235-1-Ig

Synonyms

VIRMA, MSTP054, 3F1A4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA1429 Monoclonal antibody (68235-1-Ig)

    WB analysis using 68235-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68235-1-Ig (KIAA1429 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KIAA1429 Monoclonal antibody (68235-1-Ig)

    WB analysis using 68235-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68235-1-Ig (KIAA1429 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MCF-7 cells using KIAA1429 Monoclonal antibody (68235-1-Ig)

    FC experiment of MCF-7 using 68235-1-Ig

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human KIAA1429 (68235-1-Ig, Clone:3F1A4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, 4T1 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68235-1-Ig targets KIAA1429 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KIAA1429 fusion protein Ag22449 相同性解析による交差性が予測される生物種
Full Name KIAA1429
Calculated molecular weight 1812 aa, 202 kDa
Observed molecular weight200-210 kDa
GenBank accession numberBC113380
Gene symbol KIAA1429
Gene ID (NCBI) 25962
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as oncogenic factor in breast cancer and liver cancer.

Protocols

Product Specific Protocols
WB protocol for KIAA1429 antibody 68235-1-IgDownload protocol
FC protocol for KIAA1429 antibody 68235-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols