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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIAA1429 Monoclonal antibody, PBS Only

KIAA1429 Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

3F1A4

Cat no : 68235-1-PBS

Synonyms

VIRMA, MSTP054, 3F1A4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA1429 Monoclonal antibody (68235-1-Ig)

    WB analysis using 68235-1-Ig (same clone as 68235-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68235-1-Ig (KIAA1429 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KIAA1429 Monoclonal antibody (68235-1-Ig)

    WB analysis using 68235-1-Ig (same clone as 68235-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68235-1-Ig (KIAA1429 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using KIAA1429 Monoclonal antibody (68235-1-Ig)

    FC experiment of MCF-7 using 68235-1-Ig (same clone as 68235-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human KIAA1429 (68235-1-Ig, Clone:3F1A4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68235-1-PBS targets KIAA1429 in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KIAA1429 fusion protein Ag22449 相同性解析による交差性が予測される生物種
Full Name KIAA1429
Calculated molecular weight 1812 aa, 202 kDa
Observed molecular weight200-210 kDa
GenBank accession numberBC113380
Gene symbol KIAA1429
Gene ID (NCBI) 25962
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as oncogenic factor in breast cancer and liver cancer.