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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIAA1715 Recombinant antibody, PBS Only

KIAA1715 Recombinant Antibody for ELISA, IHC, WB

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

240211C7

Cat no : 83418-4-PBS

Synonyms

LNP, Ul, Protein lunapark, LNPK, ER junction formation factor lunapark

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA1715 Recombinant antibody (83418-4-RR)

    WB analysis using 83418-4-RR (same clone as 83418-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83418-4-RR (KIAA1715 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83418-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KIAA1715 Recombinant antibody (83418-4-RR)

    IHC staining of mouse cerebellum using 83418-4-RR (same clone as 83418-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83418-4-RR (KIAA1715 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83418-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-251 cells using KIAA1715 Recombinant antibody (83418-4-RR)

    FC experiment of U-251 using 83418-4-RR (same clone as 83418-4-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug KIAA1715 Recombinant antibody (83418-4-RR, Clone:240211C7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83418-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83418-4-RR

    Affinity and Kinetic Characterization of 83418-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83418-4-RR against Human KIAA1715 were performed. The affinity constant is 13.1 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83418-4-PBS is KIAA1715 Fusion Protein expressed in E. coli.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KIAA1715 fusion protein Ag34669 相同性解析による交差性が予測される生物種
Full Name KIAA1715
GenBank accession numberBC105132
Gene symbol KIAA1715
Gene ID (NCBI) 80856
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.