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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KIAA1715 Recombinant antibody

KIAA1715 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240211C7

Cat no : 83418-4-RR

Synonyms

LNP, Ul, Protein lunapark, LNPK, ER junction formation factor lunapark

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA1715 Recombinant antibody (83418-4-RR)

    WB analysis using 83418-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83418-4-RR (KIAA1715 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using KIAA1715 Recombinant antibody (83418-4-RR)

    IHC staining of mouse cerebellum using 83418-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83418-4-RR (KIAA1715 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of U-251 cells using KIAA1715 Recombinant antibody (83418-4-RR)

    FC experiment of U-251 using 83418-4-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug KIAA1715 Recombinant antibody (83418-4-RR, Clone:240211C7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83418-4-RR

    Affinity and Kinetic Characterization of 83418-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83418-4-RR against Human KIAA1715 were performed. The affinity constant is 13.1 nM.

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Tested Applications

Positive WB detected inmouse testis tissue, mouse kidney tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83418-4-RR is KIAA1715 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KIAA1715 fusion protein Ag34669 相同性解析による交差性が予測される生物種
Full Name KIAA1715
Observed molecular weight51 kDa
GenBank accession numberBC105132
Gene symbol KIAA1715
Gene ID (NCBI) 80856
RRIDAB_3671066
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIAA1715, also known as LNPK, is a member of the conserved Lunapark protein family. KIAA1715 is an endoplasmic reticulum (ER) membrane protein consisting of an N-terminal domain important for N-myristoylation and ER targeting, two transmembrane domains and a unique zinc finger domain. It has 4 isoforms produced by alternative splicing and acts mainly as a stabilizer of negative membrane curvature (PMID: 27619977).

Protocols

Product Specific Protocols
WB protocol for KIAA1715 antibody 83418-4-RRDownload protocol
IHC protocol for KIAA1715 antibody 83418-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols