Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-82903 targets KIF15 in WB applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | KIF15 fusion protein Ag33897 相同性解析による交差性が予測される生物種 |
| Full Name | kinesin family member 15 |
| Calculated molecular weight | 160 kDa |
| Observed molecular weight | 160 kDa |
| GenBank accession number | NM_020242 |
| Gene Symbol | KIF15 |
| Gene ID (NCBI) | 56992 |
| RRID | AB_3673745 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NS87 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 KIF15 antibody CL750-82903 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |