Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, MG-63 cells, HEK-293 cells, Jurkat cells, HL-60 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, BT-549 cells, MDA-MB-468 cells |
| Positive IHC detected in | human breast cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 5 publications below |
Product Information
67190-1-Ig targets KIF20A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | KIF20A fusion protein Ag8916 相同性解析による交差性が予測される生物種 |
| Full Name | kinesin family member 20A |
| Calculated molecular weight | 890 aa, 100 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC012999 |
| Gene Symbol | KIF20A |
| Gene ID (NCBI) | 10112 |
| RRID | AB_2882485 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95235 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIF20A, also known as RAB6KIFL or MKlp2, is a kinesin originally identified as a binding partner for the rab6 GTPase involved in protein transport at the Golgi apparatus. KIF20A belongs to a large family of motor proteins that accumulate in mitotic cells and is required for normal cell division. KIF20A is thought to be involved in carcinogenesis. In normal tissues KIF20A is almost undetectable while it is overexpressed in various cancers. KIF20A has been identified as a novel promising candidate for anticancer immunotherapy.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KIF20A antibody 67190-1-Ig | Download protocol |
| IHC protocol for KIF20A antibody 67190-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Cell DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice | ||
Cancer Res Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification. | ||
Genomics Upregulation of the key biomarker kinesin family member 20A (KIF20A) is associated with the pulmonary artery hypertension | ||
medRxiv Maternal genetic variants in kinesin motor domains prematurely increase egg aneuploidy | ||
Nat Commun An oocyte meiotic midbody cap is required for developmental competence in mice |