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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIF20A Monoclonal antibody (67190-1-Ig)

    WB analysis using 67190-1-Ig (same clone as 67190-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67190-1-Ig (KIF20A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using KIF20A Monoclonal antibody (67190-1-Ig)

    WB analysis of HeLa using 67190-1-Ig (same clone as 67190-1-PBS)

    WB result of KIF20A antibody (67190-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF20A transfected HeLa cells. This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KIF20A Monoclonal antibody (67190-1-Ig)

    WB analysis using 67190-1-Ig (same clone as 67190-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67190-1-Ig (KIF20A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KIF20A Monoclonal antibody (67190-1-Ig)

    IHC staining of human breast cancer using 67190-1-Ig (same clone as 67190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KIF20A Monoclonal antibody (67190-1-Ig)

    IHC staining of human breast cancer using 67190-1-Ig (same clone as 67190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KIF20A Monoclonal antibody (67190-1-Ig)

    IHC staining of human pancreas cancer using 67190-1-Ig (same clone as 67190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using KIF20A Monoclonal antibody (67190-1-Ig)

    IHC staining of human pancreas cancer using 67190-1-Ig (same clone as 67190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67190-1-Ig (KIF20A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67190-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

KIF20A Monoclonal antibody, PBS Only

KIF20A Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67190-1-PBS
Clone No.2A8E3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

MKlp2, Mitotic kinesin-like protein 2, Kinesin-like protein KIF20A, GG10_2, FLJ21151

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67190-1-PBS targets KIF20A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KIF20A fusion protein Ag8916 相同性解析による交差性が予測される生物種
Full Name kinesin family member 20A
Calculated molecular weight 890 aa, 100 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC012999
Gene Symbol KIF20A
Gene ID (NCBI) 10112
RRIDAB_2882485
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95235
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

KIF20A, also known as RAB6KIFL or MKlp2, is a kinesin originally identified as a binding partner for the rab6 GTPase involved in protein transport at the Golgi apparatus. KIF20A belongs to a large family of motor proteins that accumulate in mitotic cells and is required for normal cell division. KIF20A is thought to be involved in carcinogenesis. In normal tissues KIF20A is almost undetectable while it is overexpressed in various cancers. KIF20A has been identified as a novel promising candidate for anticancer immunotherapy.