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  • Featured Product
  • KD/KO Validated

KIF23 Polyclonal antibody, PBS Only

KIF23 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28587-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIF23 Polyclonal antibody (28587-1-AP)

    WB analysis using 28587-1-AP (same clone as 28587-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28587-1-AP (KIF23 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using KIF23 Polyclonal antibody (28587-1-AP)

    IHC staining of human stomach cancer using 28587-1-AP (same clone as 28587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28587-1-AP (KIF23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using KIF23 Polyclonal antibody (28587-1-AP)

    IHC staining of human liver cancer using 28587-1-AP (same clone as 28587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28587-1-AP (KIF23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 28587-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KIF23 Polyclonal antibody (28587-1-AP)

    IF Staining of HepG2 using 28587-1-AP (same clone as 28587-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KIF23 antibody (28587-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28587-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28587-1-PBS targets KIF23 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF23 fusion protein Ag29346 相同性解析による交差性が予測される生物種
Full Name kinesin family member 23
Calculated molecular weight 110 kDa
Observed molecular weight110 kDa
GenBank accession numberNM_138555
Gene symbol KIF23
Gene ID (NCBI) 9493
RRIDAB_2881176
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.