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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIF23 Polyclonal antibody (28587-1-AP)

    WB analysis using 28587-1-AP (same clone as 28587-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28587-1-AP (KIF23 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using KIF23 Polyclonal antibody (28587-1-AP)

    IHC staining of human stomach cancer using 28587-1-AP (same clone as 28587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28587-1-AP (KIF23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28587-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using KIF23 Polyclonal antibody (28587-1-AP)

    IHC staining of human liver cancer using 28587-1-AP (same clone as 28587-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28587-1-AP (KIF23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 28587-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KIF23 Polyclonal antibody (28587-1-AP)

    IF Staining of HepG2 using 28587-1-AP (same clone as 28587-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KIF23 antibody (28587-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28587-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

KIF23 Polyclonal antibody, PBS Only

KIF23 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28587-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MKLP1, KNSL5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28587-1-PBS targets KIF23 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF23 fusion protein Ag29346 相同性解析による交差性が予測される生物種
Full Name kinesin family member 23
Calculated molecular weight 110 kDa
Observed molecular weight110 kDa
GenBank accession numberNM_138555
Gene Symbol KIF23
Gene ID (NCBI) 9493
RRIDAB_2881176
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02241
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

KIF23, also known as KNSL5 and MKLP1, is a member of the kinesin-like protein family. It plays a crucial role in cytokinesis, the final stage of cell division. It is involved in the formation of the central spindle, which is essential for the proper separation of the cytoplasm and the formation of the cleavage furrow. KIF23 is also involved in the organization and transport of intracellular vesicles and organelles. Mutations in KIF23 have been associated with congenital dyserythropoietic anemia type III(PMID: 23570799).