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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KIF2A Polyclonal antibody (13105-1-AP)

    WB analysis of HeLa using 13105-1-AP (same clone as 13105-1-PBS)

    WB result of KIF2A antibody (13105-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF2A transfected HeLa cells. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KIF2A Polyclonal antibody (13105-1-AP)

    WB analysis using 13105-1-AP (same clone as 13105-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13105-1-AP (KIF2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KIF2A Polyclonal antibody (13105-1-AP)

    IP experiment of mouse brain using 13105-1-AP (same clone as 13105-1-PBS)

    IP result of anti-KIF2A (IP:13105-1-AP, 4ug; Detection:13105-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KIF2A Polyclonal antibody (13105-1-AP)

    IHC staining of human breast cancer using 13105-1-AP (same clone as 13105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using KIF2A Polyclonal antibody (13105-1-AP)

    IHC staining of human breast cancer using 13105-1-AP (same clone as 13105-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using KIF2A Polyclonal antibody (13105-1-AP)

    IF Staining of A549 using 13105-1-AP (same clone as 13105-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using KIF2A antibody (13105-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using KIF2A Polyclonal antibody (13105-1-AP)

    IF Staining of MDCK using 13105-1-AP (same clone as 13105-1-PBS)

    Immunofluorescent analysis of MDCK cells using 13105-1-AP (KIF2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using KIF2A Polyclonal antibody (13105-1-AP)

    FC experiment of A549 using 13105-1-AP (same clone as 13105-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human KIF2A (13105-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 13105-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

KIF2A Polyclonal antibody, PBS Only

KIF2A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 13105-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13105-1-PBS targets KIF2A in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIF2A fusion protein Ag3748 相同性解析による交差性が予測される生物種
Full Name kinesin heavy chain member 2A
Calculated molecular weight 679 aa, 77 kDa
Observed molecular weight 80-100 kDa
GenBank accession numberBC031828
Gene Symbol KIF2A
Gene ID (NCBI) 3796
RRIDAB_2265336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00139
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.