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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KIN17 Polyclonal antibody (12313-1-AP)

    WB analysis of HeLa using 12313-1-AP

    HeLa cells, mouse testis tissue, and rat testis tissue were subjected to SDS PAGE followed by western blot with 12313-1-AP (KIN17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using KIN17 Polyclonal antibody (12313-1-AP)

    WB analysis of human heart using 12313-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12313-1-AP (KIN17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using KIN17 Polyclonal antibody (12313-1-AP)

    WB analysis of HeLa using 12313-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12313-1-AP (KIN17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KIN17 Polyclonal antibody (12313-1-AP)

    IP experiment of mouse brain using 12313-1-AP

    IP result of anti-KIN17 (IP:12313-1-AP, 4ug; Detection:12313-1-AP 1:500) with mouse brain tissue lysate 3160 ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using KIN17 Polyclonal antibody (12313-1-AP)

    IHC staining of human ovary cancer using 12313-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12313-1-AP (KIN17 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using KIN17 Polyclonal antibody (12313-1-AP)

    IF Staining of HeLa using 12313-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KIN17 antibody (12313-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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KIN17 Polyclonal antibody

KIN17 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12313-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

KIN, DNA/RNA-binding protein KIN17, DNA/RNA binding protein KIN17, BTCD, Binding to curved DNA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, human heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12313-1-AP targets KIN17 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIN17 fusion protein Ag2963 相同性解析による交差性が予測される生物種
Full Name KIN, antigenic determinant of recA protein homolog (mouse)
Calculated molecular weight 393 aa, 45 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC017309
Gene Symbol KIN17
Gene ID (NCBI) 22944
RRIDAB_2130343
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60870
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIN17 (also known as KIN) is an evolutionarily conserved zinc finger nuclear protein. It consists of three motifs, a zinc finger, a nuclear localization signal and a Kyprides-Onzonis-Woese motif that mediates transcription elongation. KIN17 is ubiquitously expressed in human tissues and participates in DNA replication, DNA repair and cell cycle progression. Emerging evidence has indicated the importance of KIN17 in the pathogenesis and progression of tumors (PMID: 28454391). KIN17 has a calculated molecular wight of 45 kDa, this antibody is raised against human KIN17 and detects a band of about 40 kDa.

Protocols

Product Specific Protocols
WB protocol for KIN17 antibody 12313-1-APDownload protocol
IHC protocol for KIN17 antibody 12313-1-APDownload protocol
IF protocol for KIN17 antibody 12313-1-APDownload protocol
IP protocol for KIN17 antibody 12313-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols