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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated KIN17 Polyclonal antibody

KIN17 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12313

Synonyms

KIN, DNA/RNA-binding protein KIN17, DNA/RNA binding protein KIN17, BTCD, Binding to curved DNA

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated KIN17 Polyclonal ant (CL488-12313)

    IF Staining of HeLa using CL488-12313

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 KIN17 antibody (CL488-12313) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated KIN17 Polyclonal ant (CL488-12313)

    FC experiment of HeLa using CL488-12313

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 KIN17 Polyclonal Antibody (CL488-12313) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12313 targets KIN17 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KIN17 fusion protein Ag2963 相同性解析による交差性が予測される生物種
Full Name KIN, antigenic determinant of recA protein homolog (mouse)
Calculated molecular weight 393 aa, 45 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC017309
Gene symbol KIN17
Gene ID (NCBI) 22944
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIN17 (also known as KIN) is an evolutionarily conserved zinc finger nuclear protein. It consists of three motifs, a zinc finger, a nuclear localization signal and a Kyprides-Onzonis-Woese motif that mediates transcription elongation. KIN17 is ubiquitously expressed in human tissues and participates in DNA replication, DNA repair and cell cycle progression. Emerging evidence has indicated the importance of KIN17 in the pathogenesis and progression of tumors (PMID: 28454391). KIN17 has a calculated molecular wight of 45 kDa, this antibody is raised against human KIN17 and detects a band of about 40 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 KIN17 antibody CL488-12313Download protocol
FC protocol for CL Plus 488 KIN17 antibody CL488-12313Download protocol
Standard Protocols
Click here to view our Standard Protocols