Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, NCI-H1299 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
66850-1-Ig targets KLF5 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | KLF5 fusion protein Ag15403 相同性解析による交差性が予測される生物種 |
| Full Name | Kruppel-like factor 5 (intestinal) |
| Calculated molecular weight | 457 aa, 51 kDa |
| Observed molecular weight | 51-57 kDa |
| GenBank accession number | BC042131 |
| Gene Symbol | KLF5 |
| Gene ID (NCBI) | 688 |
| RRID | AB_2882190 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13887 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KLF5 antibody 66850-1-Ig | Download protocol |
| FC protocol for KLF5 antibody 66850-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Targeting the KLF5-EphA2 axis can restrain cancer stemness and overcome chemoresistance in basal-like breast cancer | ||
Breast Cancer Res UCHL1 contributes to insensitivity to endocrine therapy in triple-negative breast cancer by deubiquitinating and stabilizing KLF5 | ||
BMC Nephrol Depression of LncRNA DANCR alleviates tubular injury in diabetic nephropathy by regulating KLF5 through sponge miR-214-5p | ||
Cancer Lett Energy Stress-activated AMPK Phosphorylates Snail1 and Suppresses its Stability and Oncogenic Function |