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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using KLF5 Monoclonal antibody (66850-1-Ig)

    WB analysis of HeLa using 66850-1-Ig (same clone as 66850-1-PBS)

    WB result of KLF5 antibody (66850-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KLF5 transfected HeLa cells. This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using KLF5 Monoclonal antibody (66850-1-Ig)

    WB analysis using 66850-1-Ig (same clone as 66850-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66850-1-Ig (KLF5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using KLF5 Monoclonal antibody (66850-1-Ig)

    FC experiment of HeLa using 66850-1-Ig (same clone as 66850-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human KLF5 (66850-1-Ig, Clone:2C11C5) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66850-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated
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KLF5 Monoclonal antibody, PBS Only

KLF5 Monoclonal Antibody for Indirect ELISA, WB
Cat No. 66850-1-PBS
Clone No.2C11C5

Host / Isotype

Mouse / IgG1

Reactivity

mouse, human

Applications

Indirect ELISA, WB

Synonyms

Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66850-1-PBS targets KLF5 in Indirect ELISA, WB applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KLF5 fusion protein Ag15403 相同性解析による交差性が予測される生物種
Full Name Kruppel-like factor 5 (intestinal)
Calculated molecular weight 457 aa, 51 kDa
Observed molecular weight 51-57 kDa
GenBank accession numberBC042131
Gene Symbol KLF5
Gene ID (NCBI) 688
RRIDAB_2882190
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13887
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling.