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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLHDC3 Recombinant antibody, PBS Only (Capture)

KLHDC3 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241475G5

Cat no : 84217-4-PBS

Synonyms

PEAS, Kelch domain-containing protein 3, hPEAS, 241475G5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLHDC3 Recombinant antibody (84217-4-RR)

    WB analysis using 84217-4-RR (same clone as 84217-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84217-4-RR (KLHDC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84217-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using KLHDC3 Recombinant antibody (84217-4-RR)

    FC experiment of PC-3 using 84217-4-RR (same clone as 84217-4-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug KLHDC3 Recombinant antibody (84217-4-RR, Clone:241475G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84217-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-251 cells using KLHDC3 Recombinant antibody (84217-4-RR)

    FC experiment of U-251 using 84217-4-RR (same clone as 84217-4-PBS)

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug KLHDC3 Recombinant antibody (84217-4-RR, Clone:241475G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84217-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01151-2

    Cytometric bead array standard curve of MP01151-2

    Cytometric bead array standard curve of MP01151-2, KLHDC3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84217-4-PBS. Detection antibody: 84217-2-PBS. Standard: Ag4879. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01151-3

    Cytometric bead array standard curve of MP01151-3

    Cytometric bead array standard curve of MP01151-3, KLHDC3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84217-4-PBS. Detection antibody: 84217-1-PBS. Standard: Ag4879. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84217-4-RR

    Affinity and Kinetic Characterization of 84217-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84217-4-RR against Human KLHDC3 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84217-4-PBS targets KLHDC3 as part of a matched antibody pair:

MP01151-2: 84217-4-PBS capture and 84217-2-PBS detection (validated in Cytometric bead array)

MP01151-3: 84217-4-PBS capture and 84217-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KLHDC3 fusion protein Ag4879 相同性解析による交差性が予測される生物種
Full Name kelch domain containing 3
Calculated molecular weight 382 aa, 43 kDa
Observed molecular weight43 kDa
GenBank accession numberBC041793
Gene symbol KLHDC3
Gene ID (NCBI) 116138
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The Kelch domain-containing protein 3 (KLHDC3), also designated PEAS, contains 5 Kelch repeats and may be involved in meiotic recombination process. The gene encoding KLHDC3 maps to chromosome 6, which contains around 1,200 genes within 170 million base pairs of sequence. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer suggesting the presence of a cancer susceptibility locus. Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome, 21-hydroxylase deficiency and maple syrup urine disease are also associated with genes on chromosome 6. KLHDC3 protein is mainly expressed in cytoplasm, also found in meiotic chromatin.