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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLHDC3 Recombinant antibody (84217-4-RR)

    WB analysis using 84217-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84217-4-RR (KLHDC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of PC-3 cells using KLHDC3 Recombinant antibody (84217-4-RR)

    FC experiment of PC-3 using 84217-4-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug KLHDC3 Recombinant antibody (84217-4-RR, Clone:241475G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of U-251 cells using KLHDC3 Recombinant antibody (84217-4-RR)

    FC experiment of U-251 using 84217-4-RR

    1x10^6 U-251 cells were intracellularly stained with 0.25 ug KLHDC3 Recombinant antibody (84217-4-RR, Clone:241475G5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84217-4-RR

    Affinity and Kinetic Characterization of 84217-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84217-4-RR against Human KLHDC3 were performed. The affinity constant is below 1 pM.

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KLHDC3 Recombinant antibody

KLHDC3 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84217-4-RR
Clone No.241475G5

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Synonyms

PEAS, Kelch domain-containing protein 3, hPEAS, 241475G5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNCI-H1299 cells, PC-3 cells, HepG2 cells, K-562 cells, HEK-293T cells, rat testis tissue
Positive FC (Intra) detected inPC-3 cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84217-4-RR targets KLHDC3 in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KLHDC3 fusion protein Ag4879 相同性解析による交差性が予測される生物種
Full Name kelch domain containing 3
Calculated molecular weight 382 aa, 43 kDa
Observed molecular weight43 kDa
GenBank accession numberBC041793
Gene Symbol KLHDC3
Gene ID (NCBI) 116138
RRIDAB_3671773
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BQ90
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Kelch domain-containing protein 3 (KLHDC3), also designated PEAS, contains 5 Kelch repeats and may be involved in meiotic recombination process. The gene encoding KLHDC3 maps to chromosome 6, which contains around 1,200 genes within 170 million base pairs of sequence. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer suggesting the presence of a cancer susceptibility locus. Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome, 21-hydroxylase deficiency and maple syrup urine disease are also associated with genes on chromosome 6. KLHDC3 protein is mainly expressed in cytoplasm, also found in meiotic chromatin.

Protocols

Product Specific Protocols
WB protocol for KLHDC3 antibody 84217-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols