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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLHL25 Polyclonal antibody, PBS Only

KLHL25 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27482-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLHL25 Polyclonal antibody (27482-1-AP)

    WB analysis using 27482-1-AP (same clone as 27482-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 27482-1-AP (KLHL25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27482-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KLHL25 Polyclonal antibody (27482-1-AP)

    IHC staining of mouse brain using 27482-1-AP (same clone as 27482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27482-1-AP (KLHL25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27482-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KLHL25 Polyclonal antibody (27482-1-AP)

    IHC staining of mouse brain using 27482-1-AP (same clone as 27482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27482-1-AP (KLHL25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27482-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using KLHL25 Polyclonal antibody (27482-1-AP)

    IF Staining of A549 using 27482-1-AP (same clone as 27482-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using KLHL25 antibody (27482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27482-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27482-1-PBS targets KLHL25 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLHL25 fusion protein Ag25765 相同性解析による交差性が予測される生物種
Full Name kelch-like 25 (Drosophila)
Observed molecular weight66 kDa
GenBank accession numberBC028100
Gene symbol KLHL25
Gene ID (NCBI) 64410
RRIDAB_2880883
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.