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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLHL25 Monoclonal antibody

KLHL25 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D8B8

Cat no : 67774-1-Ig

Synonyms

ENC 2, ENC2, FLJ12587, FLJ45015, kelch like 25 (Drosophila), Kelch like protein 25, KLHL25

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLHL25 Monoclonal antibody (67774-1-Ig)

    WB analysis using 67774-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67774-1-Ig (KLHL25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-29 cells using KLHL25 Monoclonal antibody (67774-1-Ig)

    WB analysis of HT-29 using 67774-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67774-1-Ig (KLHL25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KLHL25 Monoclonal antibody (67774-1-Ig)

    IHC staining of mouse testis using 67774-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67774-1-Ig (KLHL25 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KLHL25 Monoclonal antibody (67774-1-Ig)

    IHC staining of mouse testis using 67774-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67774-1-Ig (KLHL25 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, HT-29 cells, HepG2 cells, NCCIT cells, MOLT-4 cells, Raji cells, Ramos cells, Daudi cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67774-1-Ig targets KLHL25 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KLHL25 fusion protein Ag27037 相同性解析による交差性が予測される生物種
Full Name kelch-like 25 (Drosophila)
Observed molecular weight66 kDa
GenBank accession numberBC028100
Gene symbol KLHL25
Gene ID (NCBI) 64410
RRIDAB_2918539
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for KLHL25 antibody 67774-1-IgDownload protocol
IHC protocol for KLHL25 antibody 67774-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols