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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLHL25 Monoclonal antibody (67774-1-Ig)

    WB analysis using 67774-1-Ig (same clone as 67774-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67774-1-Ig (KLHL25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67774-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using KLHL25 Monoclonal antibody (67774-1-Ig)

    WB analysis of HT-29 using 67774-1-Ig (same clone as 67774-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 67774-1-Ig (KLHL25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KLHL25 Monoclonal antibody (67774-1-Ig)

    IHC staining of mouse testis using 67774-1-Ig (same clone as 67774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67774-1-Ig (KLHL25 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using KLHL25 Monoclonal antibody (67774-1-Ig)

    IHC staining of mouse testis using 67774-1-Ig (same clone as 67774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67774-1-Ig (KLHL25 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67774-1-PBS in a different storage buffer formulation.

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KLHL25 Monoclonal antibody, PBS Only

KLHL25 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67774-1-PBS
Clone No.1D8B8

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

ENC 2, ENC2, FLJ12587, FLJ45015, kelch like 25 (Drosophila), Kelch like protein 25, KLHL25

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67774-1-PBS targets KLHL25 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KLHL25 fusion protein Ag27037 相同性解析による交差性が予測される生物種
Full Name kelch-like 25 (Drosophila)
Observed molecular weight66 kDa
GenBank accession numberBC028100
Gene Symbol KLHL25
Gene ID (NCBI) 64410
RRIDAB_2918539
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H0H3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.