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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite® Plus 488-conjugated KLK11 Monoclonal ant (CL488-67427)

    IF Staining of human breast cancer using CL488-67427

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 KLK11 antibody (CL488-67427, Clone: 1E2B4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CoraLite® Plus 488-conjugated KLK11 Monoclonal ant (CL488-67427)

    IF Staining of human breast cancer using CL488-67427

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 KLK11 antibody (CL488-67427, Clone: 1E2B4 ) at dilution of 1:200.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CoraLite® Plus 488-conjugated KLK11 Monoclonal antibody

KLK11 Monoclonal Antibody for IF-P
Cat No. CL488-67427
Clone No.1E2B4

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Rat

Applications

IF-P

Synonyms

Hippostasin, hK11, Kallikrein 11, KLK11, PRSS20, Serine protease 20, TLSP, Trypsin like protease

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67427 targets KLK11 in IF-P applications and shows reactivity with Human, Pig, Rat samples.

Tested Reactivity Human, Pig, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KLK11 fusion protein Ag29895 相同性解析による交差性が予測される生物種
Full Name kallikrein-related peptidase 11
Calculated molecular weight 250 aa, 27 kDa
Observed molecular weight 30-40 kDa
GenBank accession numberBC022068
Gene Symbol KLK11
Gene ID (NCBI) 11012
RRIDAB_2919469
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UBX7
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLK11(Kallikrein-11) is also named as PRSS20, TLSP and belongs to the peptidase S1 family and Kallikrein subfamily. It is a multifunctinal protease cleaving several substrates for kallikrein and trypsin and involved in normal physiology processes in bronchus. This full length protein has a signal peptide, a propeptide and four glycosylation sites. It has 4 isoforms produced by alternative splicing with the molecular weight of 31 kDa, 27 kDa,34 kDa and 30 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 KLK11 antibody CL488-67427Download protocol
Standard Protocols
Click here to view our Standard Protocols