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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

KLK8 Monoclonal antibody

KLK8 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F3A8

Cat no : 68247-1-Ig

Synonyms

hK8, HNP, Kallikrein 8, kallikrein related peptidase 8, KLK8, Neuropsin, NP, NRPN, Ovasin, PRSS19, Serine protease 19, Serine protease TADG 14, TADG14

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLK8 Monoclonal antibody (68247-1-Ig)

    WB analysis using 68247-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of EC109 cells using KLK8 Monoclonal antibody (68247-1-Ig)

    WB analysis of EC109 using 68247-1-Ig

    EC109 cells were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using KLK8 Monoclonal antibody (68247-1-Ig)

    WB analysis of A431 using 68247-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using KLK8 Monoclonal antibody (68247-1-Ig)

    IF Staining of SH-SY5Y using 68247-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using KLK8 antibody (68247-1-Ig, Clone: 2F3A8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using KLK8 Monoclonal antibody (68247-1-Ig)

    IF Staining of SH-SY5Y using 68247-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using KLK8 antibody (68247-1-Ig, Clone: 2F3A8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, A431 cells, EC109 cells, MOLT-4 cells, NK-92 cells
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68247-1-Ig targets KLK8 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen KLK8 fusion protein Ag5642 相同性解析による交差性が予測される生物種
Full Name kallikrein-related peptidase 8
Calculated molecular weight 28 kDa
Observed molecular weight28 kDa
GenBank accession numberBC040887
Gene symbol KLK8
Gene ID (NCBI) 11202
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KLK8(Kallikrein-8) is also named as NRPN, PRSS19, TADG14.It is a new member of the human kallikrein family, which is predicted to be secreted and expected to be present in biological fluids (PMID:12507964).The protein is detectable in ovarian cancer tissue extracts, serum, and ascites fluid, indicating that it may serve as a new ovarian cancer marker (PMID:12782581). KLK8 has one predicted glycosylation site (PMID:20940292) and it has some isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for KLK8 antibody 68247-1-IgDownload protocol
IF protocol for KLK8 antibody 68247-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols