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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KLK8 Monoclonal antibody (68247-1-Ig)

    WB analysis using 68247-1-Ig (same clone as 68247-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of EC109 cells using KLK8 Monoclonal antibody (68247-1-Ig)

    WB analysis of EC109 using 68247-1-Ig (same clone as 68247-1-PBS)

    EC109 cells were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using KLK8 Monoclonal antibody (68247-1-Ig)

    WB analysis of A431 using 68247-1-Ig (same clone as 68247-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KLK8 Monoclonal antibody (68247-1-Ig)

    IHC staining of mouse brain using 68247-1-Ig (same clone as 68247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using KLK8 Monoclonal antibody (68247-1-Ig)

    IHC staining of human cervical cancer using 68247-1-Ig (same clone as 68247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using KLK8 Monoclonal antibody (68247-1-Ig)

    IHC staining of human cervical cancer using 68247-1-Ig (same clone as 68247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KLK8 Monoclonal antibody (68247-1-Ig)

    IHC staining of mouse brain using 68247-1-Ig (same clone as 68247-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using KLK8 Monoclonal antibody (68247-1-Ig)

    IF Staining of SH-SY5Y using 68247-1-Ig (same clone as 68247-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using KLK8 antibody (68247-1-Ig, Clone: 2F3A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using KLK8 Monoclonal antibody (68247-1-Ig)

    IF Staining of SH-SY5Y using 68247-1-Ig (same clone as 68247-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using KLK8 antibody (68247-1-Ig, Clone: 2F3A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68247-1-PBS in a different storage buffer formulation.

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KLK8 Monoclonal antibody, PBS Only

KLK8 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68247-1-PBS
Clone No.2F3A8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Kallikrein 8, Kallikrein-8, Kallikrein8, kallikrein related peptidase 8, EC:3.4.21.118

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68247-1-PBS targets KLK8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen KLK8 fusion protein Ag5642 相同性解析による交差性が予測される生物種
Full Name kallikrein-related peptidase 8
Calculated molecular weight 28 kDa
Observed molecular weight28 kDa
GenBank accession numberBC040887
Gene Symbol KLK8
Gene ID (NCBI) 11202
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60259
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

KLK8(Kallikrein-8) is also named as NRPN, PRSS19, TADG14.It is a new member of the human kallikrein family, which is predicted to be secreted and expected to be present in biological fluids (PMID:12507964).The protein is detectable in ovarian cancer tissue extracts, serum, and ascites fluid, indicating that it may serve as a new ovarian cancer marker (PMID:12782581). KLK8 has one predicted glycosylation site (PMID:20940292) and it has some isoforms produced by alternative splicing.