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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using KMO Recombinant antibody (83812-1-RR)

    WB analysis of MCF-7 using 83812-1-RR (same clone as 83812-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 83812-1-RR (KMO antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using KMO Recombinant antibody (83812-1-RR)

    IHC staining of mouse kidney using 83812-1-RR (same clone as 83812-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using KMO Recombinant antibody (83812-1-RR)

    IHC staining of mouse kidney using 83812-1-RR (same clone as 83812-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using KMO Recombinant antibody (83812-1-RR)

    IHC staining of mouse brain using 83812-1-RR (same clone as 83812-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using KMO Recombinant antibody (83812-1-RR)

    IHC staining of mouse heart using 83812-1-RR (same clone as 83812-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83812-1-RR (KMO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using KMO Recombinant antibody (83812-1-RR)

    FC experiment of MCF-7 using 83812-1-RR (same clone as 83812-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug KMO Recombinant antibody (83812-1-RR, Clone:240586D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83812-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83812-1-RR

    Affinity and Kinetic Characterization of 83812-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83812-1-RR against Human KMO were performed. The affinity constant is below 1 pM.

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KMO Recombinant antibody, PBS Only

KMO Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 83812-1-PBS
Clone No.240586D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

Kynurenine 3-monooxygenase, Kynurenine 3-hydroxylase, Kynurenine 3 monooxygenase, Kynurenine 3 hydroxylase, EC:1.14.13.9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83812-1-PBS targets KMO in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen KMO fusion protein Ag35029 相同性解析による交差性が予測される生物種
Full Name kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
Calculated molecular weight486 aa, 56 kDa
Observed molecular weight52-56 kDa
GenBank accession numberBC005297
Gene Symbol KMO
Gene ID (NCBI) 8564
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15229
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

KMO(Kynurenine 3-monooxygenase) is an NADPH-dependent flavin monooxygenase, catalysing the hydroxylation of the L-kynurenine to form L-3-hydroxykynurenine. KMO is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).